製品の概要

  • 製品名Anti-RPL30 antibody
    RPL30 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RPL30
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human RPL30 protein (AAH32700) with a 26kDa tag.

  • ポジティブ・コントロール
    • RPL30 transfected 293T cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa.
ELISA Use at an assay dependent dilution. Detection limit is approximately 1ng/ml as a capture antibody when used against the immunogen.

ターゲット情報

Anti-RPL30 antibody 画像

  • Anti-RPL30 antibody (ab77328) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 13 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-RPL30 antibody (ab77328) at 5 µg/ml

    Lane 1 : RPL30 transfected 293T cell lysate
    Lane 2 : Non transfected 29T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 13 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • Detection limit for recombinant tagged RPL30 is approximately 1ng/ml using ab77328 as a capture antibody.

Anti-RPL30 antibody (ab77328) 使用論文

ab77328 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab77328.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"