Anti-RPL27A 抗体 (ab74731)
Key features and details
- Rabbit polyclonal to RPL27A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL27A antibody
RPL27A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPL27A -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
synthesized peptide derived from C-terminal region of human RPL27A.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 17 kDa.
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ICC/IF | (1) |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 17 kDa. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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配列類似性
Belongs to the ribosomal protein L15P family. - Information by UniProt
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参照データベース
- Entrez Gene: 6157 Human
- Entrez Gene: 293418 Rat
- Omim: 603637 Human
- SwissProt: P46776 Human
- SwissProt: P18445 Rat
- Unigene: 523463 Human
- Unigene: 2722 Rat
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別名
- 60S ribosomal protein L27a antibody
- FLJ43464 antibody
- ribosomal protein L27a antibody
see all
画像
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All lanes : Anti-RPL27A antibody (ab74731) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of A549 cells, using ab74731 at 1/500 dilution with (+) or without (-) immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab74731 は 6 報の論文で使用されています。
- Li H et al. Loss of RPS27a expression regulates the cell cycle, apoptosis, and proliferation via the RPL11-MDM2-p53 pathway in lung adenocarcinoma cells. J Exp Clin Cancer Res 41:33 (2022). PubMed: 35073964
- Nowak RP et al. First-in-Class Inhibitors of the Ribosomal Oxygenase MINA53. J Med Chem 64:17031-17050 (2021). PubMed: 34843649
- Chen Y et al. Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes. Cell Death Differ 26:1346-1364 (2019). PubMed: 30341421
- Modelska A et al. The malignant phenotype in breast cancer is driven by eIF4A1-mediated changes in the translational landscape. Cell Death Dis 6:e1603 (2015). WB ; Human . PubMed: 25611378
- Chandra S et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci 126:3782-95 (2013). PubMed: 23750012
- Fromm-Dornieden C et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). WB . PubMed: 22436005