製品の概要

  • 製品名
    Anti-RPL23A antibody - Carboxyterminal end
    RPL23A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPL23A - Carboxyterminal end
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human RPL23A (C terminal) conjugated to keyhole limpet haemocyanin.

  • ポジティブ・コントロール
    • CEM, Hela, and HepG2 cell line lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab71341 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).

ターゲット情報

画像

  • All lanes : Anti-RPL23A antibody - Carboxyterminal end (ab71341) at 1/60 dilution

    Lane 1 : CEM cell line lysate
    Lane 2 : Hela cell line lysate
    Lane 3 : HepG2 cell line lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:
  • Datta D  et al. Nucleolar GTP-binding Protein-1 (NGP-1) Promotes G1 to S Phase Transition by Activating Cyclin-dependent Kinase Inhibitor p21 Cip1/Waf1. J Biol Chem 290:21536-52 (2015). Read more (PubMed: 26203195) »
  • Kim DH  et al. Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun 6:6843 (2015). Read more (PubMed: 25880450) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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