Rabbit polyclonal to RPL23A - Carboxyterminal end
Synthetic peptide corresponding to Human RPL23A (C terminal) conjugated to keyhole limpet haemocyanin.
CEM, Hela, and HepG2 cell line lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71341 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
This protein binds to a specific region on the 26S rRNA.
Belongs to the ribosomal protein L23P family.
N-terminus is methylated by METTL11A/NTM1.
Information by UniProt
60S ribosomal protein L23A antibody
Western blot - RPL23A antibody - Carboxyterminal end (ab71341)
All lanes :
Anti-RPL23A antibody - Carboxyterminal end (ab71341) at 1/60 dilution
Lane 1 :
CEM cell line lysate
Lane 2 :
Hela cell line lysate
Lane 3 :
HepG2 cell line lysate
Lysates/proteins at 35 µg per lane.
Predicted band size :
Observed band size :
20 kDa (
why is the actual band size different from the predicted?
Additional bands at :
25 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
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