製品の概要

  • 製品名Anti-RPL13 antibody
    RPL13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPL13
  • 特異性This antibody reacts with RPL13
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human, Drosophila melanogaster
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: KKEKARVITE EEKNFKAFAS LRMARANARL FGIRAKRAKE AAEQDVEKKK, corresponding to amino acids 162-211 of Human RPL13

  • ポジティブ・コントロール
    • Jurkat cell lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.0625 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100.

ターゲット情報

Anti-RPL13 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling RPL13 with ab50880 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar cells (mostly type I cells). Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - RPL13. Right - Merge.
  • Lane 1 :
    Lane 2 : Anti-RPL13 antibody (ab50880) at 0.0625 µg/ml

    Lane 1 : MARKER
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 24 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)

Anti-RPL13 antibody (ab50880) 使用論文

This product has been referenced in:
  • Hartl TA  et al. Regulation of ribosome biogenesis by nucleostemin 3 promotes local and systemic growth in Drosophila. Genetics 194:101-15 (2013). Read more (PubMed: 23436180) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (S2)
Specification S2
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Nov 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (3rd instar larvae)
Loading amount 50 µg
Specification 3rd instar larvae
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"