製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RPE65
  • 特異性
    This antibody do not cross react with other retinal proteins.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Monkey, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Mouse RPE65 aa 127-146.
    Sequence:

    EVTDNALVNIYPVGEDYYAC

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: >1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
    • 組織特異性
      Retinal pigment epithelium specific.
    • 関連疾患
      Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
      Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
    • 配列類似性
      Belongs to the carotenoid oxygenase family.
    • 翻訳後修飾
      Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A).
    • 細胞内局在
      Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated.
    • Information by UniProt
    • 参照データベース
    • 別名
      • All-trans-retinyl-palmitate hydrolase antibody
      • LCA 2 antibody
      • LCA2 antibody
      • Leber congenital amaurosis antibody
      • mRPE 65 antibody
      • mRPE65 antibody
      • p63 antibody
      • rd 12 antibody
      • rd12 antibody
      • Retinal pigment epithelium specific 61 kDa protein antibody
      • Retinal pigment epithelium specific 65 kDa protein antibody
      • Retinal pigment epithelium specific protein antibody
      • Retinal pigment epithelium specific protein 65kDa antibody
      • Retinal pigment epithelium-specific 65 kDa protein antibody
      • Retinitis pigmentosa 20 antibody
      • Retinoid isomerohydrolase antibody
      • Retinol isomerase antibody
      • RP 20 antibody
      • RP20 antibody
      • RPE 65 antibody
      • RPE65 antibody
      • RPE65_HUMAN antibody
      • sRPE 65 antibody
      • sRPE65 antibody
      see all

    プロトコール

    参考文献

    ab74998 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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