Anti-RPE65 抗体 (ab67042)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RPE65
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Chicken, Cow, Human, Xenopus laevis, Non human primates, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human RPE65.

    (Peptide available as ab99880.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the Mouse Retina Tissue Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).

ターゲット情報

  • 機能
    Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
  • 組織特異性
    Retinal pigment epithelium specific.
  • 関連疾患
    Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
    Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
  • 配列類似性
    Belongs to the carotenoid oxygenase family.
  • 翻訳後修飾
    Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A).
  • 細胞内局在
    Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • All-trans-retinyl-palmitate hydrolase antibody
    • LCA 2 antibody
    • LCA2 antibody
    • Leber congenital amaurosis antibody
    • mRPE 65 antibody
    • mRPE65 antibody
    • p63 antibody
    • rd 12 antibody
    • rd12 antibody
    • Retinal pigment epithelium specific 61 kDa protein antibody
    • Retinal pigment epithelium specific 65 kDa protein antibody
    • Retinal pigment epithelium specific protein antibody
    • Retinal pigment epithelium specific protein 65kDa antibody
    • Retinal pigment epithelium-specific 65 kDa protein antibody
    • Retinitis pigmentosa 20 antibody
    • Retinoid isomerohydrolase antibody
    • Retinol isomerase antibody
    • RP 20 antibody
    • RP20 antibody
    • RPE 65 antibody
    • RPE65 antibody
    • RPE65_HUMAN antibody
    • sRPE 65 antibody
    • sRPE65 antibody
    see all

Anti-RPE65 antibody 画像

  • Anti-RPE65 antibody (ab67042) at 1 µg/ml + Mouse Retina Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • All lanes : Anti-RPE65 antibody (ab67042) at 1/1000 dilution

    Lane 1 : Lepomis macrochirus (blue gill)RPE Lysate at 10 µl
    Lane 2 : Lepomis macrochirus (blue gill)RPE Lysate at 5 µl

    Secondary
    HRP congugated Goat anti-rabbit at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

プロトコール

Anti-RPE65 antibody (ab67042) 使用論文

ab67042 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Rat Tissue lysate - whole (Retina, RPE)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina, RPE
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Jul 25 2017

Application
Western blot
Sample
Human Tissue lysate - whole (Cultured human fetal retinal pigment epithelium ce)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Treatment
Previously extracted for metabolites with 80% methanol
Specification
Cultured human fetal retinal pigment epithelium ce
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Jun 20 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Retinal pigment epithlium, retina, eye cup)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retinal pigment epithlium, retina, eye cup
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Dec 12 2016

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Retina extracted w/ M-PER)
Gel Running Conditions
Reduced Denaturing (10%, hand-cast)
Loading amount
35 µg
Specification
Retina extracted w/ M-PER
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Jun 03 2016

Application
Western blot
Sample
Fish Cell lysate - whole cell (retinal pigment epithelium)
Loading amount
20 µg
Specification
retinal pigment epithelium
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 12 2013

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