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Bovine RPE microsomal membranes.
Our Abpromise guarantee covers the use of ab78036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration.|
|Other||Use at an assay dependent concentration.|
|WB||1/5000 - 1/20000. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||1/250 - 1/500.|
Immunocytochemistry/ Immunofluorescence analysis of rat glioblastoma cell line C6 cells labeling RPE65 with ab78036 at 1/50 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0,1% Triton X 100 in PBS. The cells were blocked with 0.5% BSA for 30 minutes at room temperature, followed by incubation with ab78036 for 16 hours in 0.5% BSA in PBS at 4°C. A goat anti-mouse Cy3® secondary antibody was used at 1/400 dilution.
ab78036 staining RPE65 in Rhesus monkey retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.