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Immunogen was a synthetic peptide, which represented a portion of human DNA directed RNA polymerase II 140 kDa subunit encoded within exon 4 (LocusLink ID 5431).
Our Abpromise guarantee covers the use of ab10332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
Detection of RPB2 using ab10332 by Western blot. Samples: Whole cell lysate (50 mg) from 293 cells. From left to right: ab10332 concentrations 1, 0.5, 0.5 and 2mg/ml; two left hand columns after 1 minute’s exposure and two right hand columns after 2 hours’ exposure. Band representing RPB2 at approximately 140kDa.
Detection of RPB2 using ab10332 by immunoprecipitation. Samples: Whole cell lysate from 293 cells. Antibodies: ab10332 used at 10mg/10cm IP plate. Detection: chemiluminescence with exposure time of 10 seconds.