製品の概要

  • 製品名Anti-RPA70 antibody
    RPA70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPA70
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 369 and 616 of Human RPA70 (NP_002936).

  • ポジティブ・コントロール
    • 293T, A431, HepG2, Molt-4, and Raji cell lysates. H1299 and HeLaS3 whole cell lysate. HeLa cells for IF. Cal27 xenograft for IHC.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • 配列類似性Belongs to the replication factor A protein 1 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dmrpa1 antibody
    • Drosophila Replication Protein A antibody
    • DRPA antibody
    • HSSB antibody
    • Human single stranded DNA binding protein antibody
    • MST075 antibody
    • MSTP075 antibody
    • p70 antibody
    • REPA1 antibody
    • Replication factor A antibody
    • Replication factor A protein 1 antibody
    • Replication protein A 70 kDa DNA-binding subunit antibody
    • Replication protein A 70kDa DNA binding subunit antibody
    • Replication protein A1 70kDa antibody
    • Replication protein A1 antibody
    • RF A antibody
    • RF-A protein 1 antibody
    • RFA antibody
    • RFA1_HUMAN antibody
    • RP A antibody
    • RP-A p70 antibody
    • RPA 70 antibody
    • RPA antibody
    • rpa1 antibody
    • Single stranded binding protein 70 antibody
    • Single-stranded DNA-binding protein antibody
    see all

Anti-RPA70 antibody 画像

  • All lanes : Anti-RPA70 antibody (ab97338) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 68 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA70 antibody (ab97338) at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA70 antibody (ab97338) at 1/100 dilution.

Anti-RPA70 antibody (ab97338) 使用論文

This product has been referenced in:
  • Marin-Vicente C  et al. RRP6/EXOSC10 is required for the repair of DNA double-strand breaks by homologous recombination. J Cell Sci 128:1097-107 (2015). Read more (PubMed: 25632158) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"