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Immunogen was a synthetic peptide, which represented a portion of human replication protein A1, 70kDa encoded within exon 12 (LocusLink ID 6117).
Our Abpromise guarantee covers the use of ab12320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 3-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/5000 - 1/25000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
Detection of Human RPA70 by Western Blot.