製品の概要

  • 製品名Anti-RPA34 antibody [9H8 / 34A / alpha SSB34A]
  • 製品の詳細
    Mouse monoclonal [9H8 / 34A / alpha SSB34A] to RPA34
  • 特異性We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified human RPA34 protein.

  • ポジティブ・コントロール
    • HeLa cells.
  • 特記事項


    ab81191 inhibits the stimulation of pol alpha, but has no effect on DNA unwinding and pol delta stimulation (Kenny et. al., 1990).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 29 kDa. For 2 hours at room temperature.
IP Use at 2 µg/mg of lysate. Native verified.
EMSA Use at an assay dependent concentration.

ターゲット情報

  • 関連性Replication Protein A (RPA) is a single stranded DNA binding protein. RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
  • 細胞内局在Nuclear. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • 参照データベース
  • 別名
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • replication protein A2 (32kD) antibody
    • replication protein A2, 32kDa antibody
    • RF A protein 2 antibody
    • RP A p32 antibody
    • RPA2 antibody
    • RPA32 antibody
    see all

Anti-RPA34 antibody [9H8 / 34A / alpha SSB34A] 画像

  • anti RPA34 (version of ab81191 containing BSA and Azide) at 1 µg/ml + human cells

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-RPA34 antibody [9H8 / 34A / alpha SSB34A] (ab81191) 使用論文

This product has been referenced in:
  • Kenny MK  et al. The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J Biol Chem 265:7693-700 (1990). WB . Read more (PubMed: 2159011) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"