Synthetic peptide within Human RPA32/RPA2. The exact sequence is proprietary. Synthesized phosphopeptide derived from human RPA32/RFA2 around the phosphorylation site of threonine 21(G-Y-TP-Q-S). Database link: P15927
IHC-P: Human lung carcinoma tissue.
WB: Adriamycin treated HeLa cell lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
ab61065 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 22773103
1/50 - 1/100.
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
RCJMB04_6d17 replication protein A2, 32kDa antibody
Replication factor A protein 2 antibody
Replication protein A 32 kDa subunit antibody
Replication protein A 32kDa subunit antibody
Replication protein A 34 kDa subunit antibody
Replication protein A antibody
Replication Protein A2 (32kDa) antibody
Replication protein A2 antibody
Replication protein A2, 32kDa antibody
RF-A protein 2 antibody
RP-A p32 antibody
RP-A p34 antibody
RPA 2 antibody
RPA 32 antibody
Western blot - Anti-RPA32/RPA2 (phospho T21) antibody (ab61065)
All lanes : Anti-RPA32/RPA2 (phospho T21) antibody (ab61065) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysates treated with Adriamycin 0.5 µg/ml 24 hours with immunizing phosphopeptide