製品の概要

  • 製品名Anti-RPA32/RPA2 antibody
    RPA32/RPA2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPA32/RPA2
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 172 of human RPA32/RPA2 (NP_002937)

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates A549 xenograft HeLa cells

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • 翻訳後修飾Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • 細胞内局在Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • 参照データベース
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Anti-RPA32/RPA2 antibody 画像

  • All lanes : Anti-RPA32/RPA2 antibody (ab97594) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
  • ab97594, at a 1/100 dilution, staining human RPA32/RPA2 in A549 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97594 at a 1/200 dilution (top image) and merged with DNA probe (bottom image).

Anti-RPA32/RPA2 antibody (ab97594) 使用論文

ab97594 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"