Anti-RPA14/RPA3 抗体 (ab97436)
Key features and details
- Rabbit polyclonal to RPA14/RPA3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPA14/RPA3 antibody
RPA14/RPA3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPA14/RPA3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow -
免疫原
Recombinant fragment corresponding to Human RPA14/RPA3 aa 1-91.
Database link: NP_002938 -
ポジティブ・コントロール
- WB: H1299, HeLaS3, MOLT4 and HepG2 whole cell lysate (ab7900) IHC-P: Human lung CA patient tumor
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97436の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/100 - 1/250.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa. |
IHC-P
1/100 - 1/250. |
ターゲット情報
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機能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6119 Human
- Omim: 179837 Human
- SwissProt: P35244 Human
- Unigene: 487540 Human
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別名
- p14 antibody
- REPA 3 antibody
- REPA3 antibody
see all
画像
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All lanes : Anti-RPA14/RPA3 antibody (ab97436) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa -
Immunohistochemical analysis of RPA14/RPA3 in paraffin embedded lung CA patient tumor, using ab97436 at a 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97436 は 2 報の論文で使用されています。
- Pedersen H et al. Replication Protein A (RPA) Mediates Radio-Resistance of Glioblastoma Cancer Stem-Like Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32111042
- Yenerall P et al. RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Cell Chem Biol 27:105-121.e14 (2020). PubMed: 31883965