製品の概要

  • 製品名Anti-RPA14 antibody [11.1]
    RPA14 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [11.1] to RPA14
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    N-terminally 6x-His tagged fusion protein encoding full-length human RPA-14 expressed in E. coli.

  • ポジティブ・コントロール
    • HeLa, Raji, NIH 3T3cells and recombinant protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 2 µg/ml. Predicted molecular weight: 14 kDa.
IP Use a concentration of 0.1 - 2 µg/ml.
Flow Cyt Use 1µg for 106 cells.
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
      Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • p14 antibody
      • REPA 3 antibody
      • REPA3 antibody
      • Replication factor A protein 3 antibody
      • Replication protein A 14 kDa subunit antibody
      • Replication protein A3 14kD antibody
      • Replication protein A3 14kDa antibody
      • Replication protein A3 antibody
      • RF A antibody
      • RF A protein 3 antibody
      • RF-A protein 3 antibody
      • RFA protein 3 antibody
      • RFA3_HUMAN antibody
      • RP A antibody
      • RP A p14 antibody
      • RP-A p14 antibody
      • RPA 14 antibody
      • RPA 3 antibody
      • RPA p14 antibody
      • rpa3 antibody
      see all

    Anti-RPA14 antibody [11.1] 画像

    • Overlay histogram showing K562 cells stained with ab588 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab588, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-RPA14 antibody [11.1] (ab588) 使用論文

    ab588 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Hela, 293, SupT1, Jurkat, primary CD4+)
    Specification Hela, 293, SupT1, Jurkat, primary CD4+
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 0.5
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    Verified customer

    投稿 Aug 22 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (hela cells)
    Total protein in input 5 µg
    Specification hela cells
    Immuno-precipitation step Protein A/G
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    Verified customer

    投稿 Jul 31 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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    Verified customer

    投稿 Aug 22 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"