製品の概要

  • 製品名Anti-ROS1 antibody
    ROS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ROS1
  • アプリケーション適用あり: ICC/IF, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human ROS1 aa 23-56 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: P08922

  • ポジティブ・コントロール
    • HL-60 cell lysate.
  • 特記事項

    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • 一次抗体 備考Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 258 kDa (predicted molecular weight: 281 kDa).

ターゲット情報

  • 機能This is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity.
  • 組織特異性Expressed in brain. Expression is increased in primary gliomas.
  • 関連疾患Note=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 9 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c ros 1 antibody
    • c ros antibody
    • c ros oncogene 1 receptor tyrosine kinase antibody
    • c Ros receptor tyrosine kinase antibody
    • c ros1 antibody
    • MCF 3 antibody
    • MCF3 antibody
    • Oncogene ROS antibody
    • OTTHUMP00000017814 antibody
    • OTTHUMP00000017815 antibody
    • OTTHUMP00000040389 antibody
    • Proto oncogene c ros 1 protein antibody
    • Proto oncogene c Ros antibody
    • Proto oncogene tyrosine protein kinase ROS antibody
    • Proto oncogene tyrosine protein kinase ROS precursor antibody
    • Proto-oncogene c-Ros-1 antibody
    • Proto-oncogene tyrosine-protein kinase ROS antibody
    • Receptor tyrosine kinase c ros oncogene 1 antibody
    • Ros 1 antibody
    • ROS 1C antibody
    • ROS antibody
    • ROS proto oncogene 1 , receptor tyrosine kinase antibody
    • ROS_HUMAN antibody
    • ROS1 antibody
    • ROS1C antibody
    • Transmembrane tyrosine specific protein kinase antibody
    • v ros avian UR2 sarcoma virus oncogene homolog 1 antibody
    • v ros UR2 sarcoma virus oncogene homolog 1 antibody
    see all

Anti-ROS1 antibody 画像



  • Predicted band size : 281 kDa
    ab5512 at a 1/100 dilution staining approximately 258kDa band of ROS in HL-60 cell lysate by Western blot (ECL). ab5512 at a 1/100 dilution staining approximately 258kDa band of ROS in HL-60 cell lysate by Western blot (ECL).
  • ab5512 staining ROS1 in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ICC/IF image of ab5512 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5512, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ROS1 antibody (ab5512) 使用論文

This product has been referenced in:
  • Song SJ  et al. Aurora A regulates prometaphase progression by inhibiting the ability of RASSF1A to suppress APC-Cdc20 activity. Cancer Res 69:2314-23 (2009). IHC ; Human . Read more (PubMed: 19276349) »
  • Jun HJ  et al. Epigenetic regulation of c-ROS receptor tyrosine kinase expression in malignant gliomas. Cancer Res 69:2180-4 (2009). IHC-P ; Human . Read more (PubMed: 19276365) »

See all 2 Publications for this product

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