製品の概要

  • 製品名Anti-ROR1 antibody [2H6]
    ROR1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2H6] to ROR1
  • アプリケーション適用あり: Flow Cyt, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to extracellular amino acids 30-406 of Human ROR1 fused with hIgGFc tag, expressed in HEK293 cells.

  • ポジティブ・コントロール
    • Recombinant extracellular fragment (aa30-406) of Human ROR1 and HEK293 cells transfected with extracellular ROR1 (aa30-406)-hIgGFc.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 104 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

ターゲット情報

  • 機能Tyrosine-protein kinase receptor whose role is not yet clear.
  • 組織特異性Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
    Contains 1 FZ (frizzled) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 kringle domain.
    Contains 1 protein kinase domain.
  • 発生段階Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ537F10.1 antibody
    • MGC99659 antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor related 1 antibody
    • Neurotrophic tyrosine kinase, receptor related 1 antibody
    • NTRKR1 antibody
    • OTTHUMP00000010573 antibody
    • OTTHUMP00000010574 antibody
    • OTTMUSP00000008344 antibody
    • Receptor tyrosine kinase like orphan receptor 1 antibody
    • receptor-related 1 antibody
    • RGD1559469 antibody
    • ROR 1 antibody
    • ROR1 antibody
    • ROR1_HUMAN antibody
    • RP11 24J23.1 antibody
    • Tyrosine kinase like orphan receptor 1 antibody
    • Tyrosine protein kinase transmembrane receptor ROR1 antibody
    • Tyrosine-protein kinase transmembrane receptor ROR1 antibody
    see all

Anti-ROR1 antibody [2H6] 画像

  • Anti-ROR1 antibody [2H6] (ab91187) at 1/500 dilution + recombinant extracellular fragment (aa30-406) of Human ROR1

    Predicted band size : 104 kDa
  • ab91187 at 1/200 dilution staining ROR1 in HEK293 cells transfected with extracellular ROR1 (aa30-406)-hIgGFc by Confocal Immunofluorescence (green). DRAQ5 fluorescent DNA dye (blue).
  • Overlay histogram showing A549 cells stained with ab91187 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91187, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-ROR1 antibody [2H6] (ab91187) 使用論文

This product has been referenced in:
  • Weekes MP  et al. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157:1460-72 (2014). Flow Cyt ; Human . Read more (PubMed: 24906157) »

See 1 Publication for this product

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