製品の概要

  • 製品名Anti-ROM1 antibody
    ROM1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to ROM1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human ROM1 protein (NP_000318.1)

  • ポジティブ・コントロール
    • Human kidney lysate; ROM1 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.

ターゲット情報

  • 機能May function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. It is essential for disk morphogenesis.
  • 組織特異性Retina (photoreceptor). In rim region of ROS (rod outer segment) disks.
  • 関連疾患Defects in ROM1 may cause retinitis pigmentosa (RP) [MIM:268000]; when associated with defects in PRPH2.
  • 配列類似性Belongs to the PRPH2/ROM1 family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Retinal outer segment membrane protein 1 antibody
    • Rod outer segment membrane protein 1 antibody
    • ROM antibody
    • ROM1 antibody
    • ROM1_HUMAN antibody
    • ROSP1 antibody
    • RP7 antibody
    • Tetraspanin 23 antibody
    • Tetraspanin-23 antibody
    • Tspan 23 antibody
    • Tspan-23 antibody
    • TSPAN23 antibody
    see all

Anti-ROM1 antibody 画像

  • Anti-ROM1 antibody (ab72973) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-ROM1 antibody (ab72973) at 1/500 dilution

    Lane 1 : ROM1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-ROM1 antibody (ab72973) 使用論文

ab72973 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step normal goat serum (NGS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Cow Cell (bovine rom1 ortholog expressin in AD293)
Specification bovine rom1 ortholog expressin in AD293
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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投稿 Aug 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"