製品の概要

  • 製品名Anti-Robo2 antibody
    Robo2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Robo2
  • 特異性ab75014 has shown to not cross react with human Robo1 or Robo3 protein.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1250 - 1350 of Mouse Robo2.

    (Peptide available as ab75013.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Rat Liver Mouse Liver

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 161 kDa).Can be blocked with Mouse Robo2 peptide (ab75013).

ターゲット情報

  • 機能Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
  • 関連疾患Defects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2) [MIM:610878]. VUR is a complex, genetically heterogeneous developmental disorder characterized by the retrograde flow of urine from the bladder into the ureter and is associated with reflux nephropathy, the cause of 15% of end-stage renal disease in children and young adults.
    Note=A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. Translocation t(Y;3)(p11;p12) with PCDH11Y. This translocation disrupts ROBO2 and produces dominant-negative ROBO2 proteins that abrogate SLIT-ROBO signaling in vitro.
  • 配列類似性Belongs to the immunoglobulin superfamily. ROBO family.
    Contains 3 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • lea antibody
    • Robo 2 antibody
    • Robo2 antibody
    • ROBO2_HUMAN antibody
    • Roundabout 2 antibody
    • Roundabout homolog 2 antibody
    • roundabout, axon guidance receptor, homolog 2 (Drosophila) antibody
    • Roundabout2 antibody
    • SAX 3 antibody
    • SAX3 antibody
    see all

Anti-Robo2 antibody 画像

  • Lanes 1 - 3 : Anti-Robo2 antibody (ab75014) at 2 µg/ml
    Lanes 4 - 6 : Mouse Monoclonal to V5 at 1/1000 dilution

    Lane 1 : HEK293 cells expressing Robo1
    Lane 2 : HEK293 cells expressing Robo2-V5
    Lane 3 : HEK293 cells expressing Robo3
    Lane 4 : HEK293 cells expressing Robo1
    Lane 5 : HEK293 cells expressing Robo2-V5
    Lane 6 : HEK293 cells expressing Robo3

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 3 : Anti-Rabbit HRP at 1/20000 dilution
    Lanes 4 - 6 : Anti-Mouse HRP


    Predicted band size : 161 kDa

    This image is courtesy of Alain Chedotal (Institut Vision, France)

  • All lanes : Anti-Robo2 antibody (ab75014) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 161 kDa


    Exposure time : 20 minutes

Anti-Robo2 antibody (ab75014) 使用論文

ab75014 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E13.5 midbrains, 5 microns)
Specification E13.5 midbrains, 5 microns
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
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Abcam user community

Verified customer

投稿 May 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (9L, PC12, ms Brain)
Loading amount 10 µg
Specification 9L, PC12, ms Brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Mr. Antibody Solutions

Verified customer

投稿 Apr 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (9L, PC12, ms Brain)
Loading amount 10 µg
Specification 9L, PC12, ms Brain
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Mar 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"