Ro 31-8220 mesylate (Bisindolylmaleimide IX) (ab120374)
- データシート
- 使用論文 (1)
製品の概要
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製品名Ro 31-8220 mesylate (Bisindolylmaleimide IX)
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製品の詳細Protein kinase inhibitor
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別名
- Bisindolylmaleimide IX
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生理活性の詳細Widely used as a cell-permeable, reversible, competitive protein kinase C inhibitor. Also potently inhibits wide range of kinases inlcuding RSK1, RSK2, PRK2, PKCα, PKD1, MSK1, GSK3β, CDK2-cyclin A and PIM3, as well as PKCα.
Also available in simple stock solutions (ab146723) - add 1 ml of water to get an exact, ready-to-use concentration. -
精製度> 98%
製品の特性
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体系名3-[1-[3-(Amidinothio)propyl]-3-indolyl]-4-(1-methyl-3-indolyl)-1H-pyrrole-2,5-dione methanesulfonate
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分子量553.65
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構造式
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分子式C25H23N5O2S.CH3SO3H
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CAS 番号125314-64-9
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PubChem 登録番号11628205
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保存方法Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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溶解性Soluble in water to 100 mM, DMSO to 100 mM and in ethanol to 5 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記CS(=O)(=O)O.Cn2cc(c1ccccc12)C=5C(=O)NC(=O)C=5c4cn(CCCSC(=N)N)c3ccccc34
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由来
Synthetic
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研究分野
画像
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Functional Studies - Ro 31-8220 mesylate (Bisindolylmaleimide IX) (ab120374)1.5 x 10e7 THP-1 cells were incubated with 100 nM GF 109203X (ab144264) or Ro 31-8220 mesylate (ab120374) for 30 minutes prior to activation with 10 µg x mL-1 PMA (Sigma) for 4 hours. Control cells were left without inhibitors or PMA. Cells were lysed in 1 mL of lysis buffer, and 30 µL were tested for PKC activity (duplicates; +/- SD).
参考文献
This product has been referenced in:
- Bain J et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
- Davis PD et al. Inhibitors of protein kinase C. 2. Substituted bisindolylmaleimides with improved potency and selectivity. J Med Chem 35:994-1001 (1992). Read more (PubMed: 1552513) »
- Dieter P & Fitzke E RO 31-8220 and RO 31-7549 show improved selectivity for protein kinase C over staurosporine in macrophages. Biochem Biophys Res Commun 181:396-401 (1991). Read more (PubMed: 1958208) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"