製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RNF8
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    GEPGFFVTGDRAG-C

    , corresponding to N terminal amino acids 2-14 of Human RNF8. (Peptide available as ab23278.)

  • ポジティブ・コントロール
    • Human lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).Can be blocked with Human RNF8 peptide (ab23278).

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.
  • 組織特異性
    Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the RNF8 family.
    Contains 1 FHA domain.
    Contains 1 RING-type zinc finger.
  • 発生段階
    Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase.
  • ドメイン
    The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2.
  • 翻訳後修飾
    Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
  • 細胞内局在
    Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C3HC4 type zinc finger protein antibody
    • E3 ubiquitin protein ligase RNF8 antibody
    • E3 ubiquitin-protein ligase RNF8 antibody
    • FLJ12013 antibody
    • KIAA0646 antibody
    • Ring finger protein (C3HC4 type) 8 antibody
    • RING finger protein 8 antibody
    • Ring finger protein 8, E3 ubiquitin protein ligase antibody
    • RNF 8 antibody
    • RNF8 antibody
    • RNF8_HUMAN antibody
    • UBC13/UEV interacting ring finger protein antibody
    see all

Anti-RNF8 antibody 画像

  • Anti-RNF8 antibody (ab15850) at 0.1 µg/ml + Lysates prepared from human lung tissues at 35 µg

    Predicted band size : 56 kDa
  • ab15850 staining RNF8 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using NP40, blocked with 5% BSA fo 1 hour at 25°C and then incubated with ab15850 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-goat polyclonal used at a 1/500 dilution.

    See Abreview

Anti-RNF8 antibody (ab15850) 使用論文

ab15850 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Formaldehyde
Permeabilization
Yes - NP40
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Andrew Cobb

Verified customer

投稿 Mar 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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