Anti-RNF8 抗体 (ab105362)

製品の概要

• 製品名
  Anti-RNF8 antibody
  RNF8 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to RNF8
• アプリケーション
  適用あり: WB, ICC/IFmore details
• 種交差性
  交差種: Mouse, Rat, Human
  
• 免疫原

  A 14 amino acid peptide near the C terminus of Human RNF8 (NP_003949).

• ポジティブ･コントロール
  • WB: Human lung tissue lysate ICC/IF: Rat lung tissue

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• バッファー
  Preservative: 0.02% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• 精製度
  Immunogen affinity purified
• ポリ/モノ
  ポリクローナル
• アイソタイプ
  IgG
• 研究分野
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E3 Enzymes
  • RING Finger E3 Ligase

  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • E3 Ubiquitin Ligases

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

アプリケーション

ターゲット情報

• 機能
  E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.
• 組織特異性
  Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.
• パスウェイ
  Protein modification; protein ubiquitination.
• 配列類似性
  Belongs to the RNF8 family.
  Contains 1 FHA domain.
  Contains 1 RING-type zinc finger.
• 発生段階
  Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase.
• ドメイン
  The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2.
• 翻訳後修飾
  Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
• 細胞内局在
  Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.

• Target information above from: UniProt accession O76064 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 9025 Human
  • Entrez Gene: 58230 Mouse
  • Entrez Gene: 361815 Rat
  • Omim: 611685 Human
  • SwissProt: O76064 Human
  • SwissProt: Q8VC56 Mouse
  • SwissProt: Q4KLN8 Rat
  • Unigene: 485278 Human

  • Unigene: 305994 Mouse
  • Unigene: 12463 Rat

  see all
• 別名
  • C3HC4 type zinc finger protein antibody
  • E3 ubiquitin protein ligase RNF8 antibody
  • E3 ubiquitin-protein ligase RNF8 antibody

  • FLJ12013 antibody
  • KIAA0646 antibody
  • Ring finger protein (C3HC4 type) 8 antibody
  • RING finger protein 8 antibody
  • Ring finger protein 8, E3 ubiquitin protein ligase antibody
  • RNF 8 antibody
  • RNF8 antibody
  • RNF8_HUMAN antibody
  • UBC13/UEV interacting ring finger protein antibody

  see all

プロトコール

• Western blot protocols
• Immunocytochemistry & immunofluorescence protocols