製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF6
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Cynomolgus monkey, Orangutan
  • 免疫原

    Synthetic peptide: NLVETGTLPI LRLAHFFLLN ESDDDDRIRG LTKEQIDNLS TRHYEHNSID, corresponding to amino acids 576-625 of Human RNF6 (NP_005968)

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Can be blocked with RNF6 peptide (ab195206). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.
  • 組織特異性
    Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 関連疾患
    Defects in RNF6 may be a cause of esophageal cancer (ESCR) [MIM:133239]. A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage.
  • 配列類似性
    Belongs to the RNF12 family.
    Contains 1 RING-type zinc finger.
  • 発生段階
    Expressed in embryo and adult testis.
  • 細胞内局在
    Nucleus. Cytoplasm. Cell projection > axon. Nucleus > PML body. Localizes to the PML nuclear bodies in Sertoli cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase RNF6 antibody
    • Ring finger protein (C3H2C3 type) 6 antibody
    • Ring finger protein 6 antibody
    • RING H2 protein antibody
    • RING H2 protein RNF6 antibody
    • RNF 6 antibody
    • RNF6 antibody
    • RNF6_HUMAN antibody
    • SPG2 antibody
    see all

画像

  • Anti-RNF6 antibody (ab80427) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa

プロトコール

参考文献

This product has been referenced in:
  • Devkota S  et al. Functional characterization of EI24-induced autophagy in the degradation of RING-domain E3 ligases. Autophagy 12:2038-2053 (2016). Read more (PubMed: 27541728) »

See 1 Publication for this product

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