製品の概要

  • 製品名Anti-RNF43 antibody
  • 製品の詳細
    Rabbit polyclonal to RNF43
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide designed within residues: DFDPLVYCSP KGDPQRVDMQ PSVTSRPRSL DSVVPTGETQ VSSHVHYHRH, corresponding to internal sequence amino acids 504 - 553 of Human RNF43 (NP_060233)

  • ポジティブ・コントロール
    • MCF7 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab84125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性RNF43 is a probable E3 ubiquitin-protein ligase that promotes cell growth and is upregulated in colon cancer. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
  • 細胞内局在Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note: may be secreted.
  • 参照データベース
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Anti-RNF43 antibody 画像

  • Anti-RNF43 antibody (ab84125) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab84125 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RNF43 antibody (ab84125) 使用論文

ab84125 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Gel Running Conditions Reduced Denaturing
Loading amount 5 µg
Specification mouse embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"