製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF34
  • 特異性
    Expect reactivity with isoform 1 and 2 of RNF34.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • RNF34 transfected lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    Purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the given buffer.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
  • 組織特異性
    Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 FYVE-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 2 SAP domains.
  • ドメイン
    The RING-type zinc finger is required for the ubiquitination of target proteins.
    The FYVE-type zinc finger domain is required for localization and may confer affinity for cellular compartments enriched in phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate phospholipids.
  • 翻訳後修飾
    Autoubiquitinated (in vitro).
    Proteolytically cleaved by caspases upon induction of apoptosis by TNF.
  • 細胞内局在
    Cell membrane. Endomembrane system. Nucleus. Nucleus speckle. Cytoplasm, cytosol.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CARP 1 antibody
    • CARP-1 antibody
    • CARP1 antibody
    • Caspase regulator CARP1 antibody
    • Caspases-8 and -10-associated RING finger protein 1 antibody
    • E3 ubiquitin-protein ligase RNF34 antibody
    • FYVE-RING finger protein Momo antibody
    • hRFI antibody
    • Human RING finger homologous to inhibitor of apoptosis protein antibody
    • RFI antibody
    • RIF antibody
    • RIFF antibody
    • RING finger protein 34 antibody
    • RING finger protein RIFF antibody
    • RNF34 antibody
    • RNF34_HUMAN antibody
    see all

画像

  • All lanes : Anti-RNF34 antibody (ab47255) at 1/1000 dilution

    Lane 1 : Empty vector 293T cell lysate
    Lane 2 : RNF34 transfected 293T cell lysate
    Lane 3 : RFFL transfected 293T cell lysate

    Lysates/proteins at 20 µg/ml per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa

プロトコール

参考文献

ab47255 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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