Anti-RNF31/HOIP 抗体 - ChIP Grade (ab46322)

製品の概要

  • 製品名
    Anti-RNF31/HOIP antibody - ChIP Grade
    RNF31/HOIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RNF31/HOIP - ChIP Grade
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WB, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Human RNF31/HOIP.

    (Peptide available as ab46321.)

  • ポジティブ・コントロール
    • ab46322 gave a positive signal in Jurkat Whole Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ChIP
  • 追加情報
    ChIP: Use at an assay dependent dilution (PMID 19237537.
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: 1/250. Detects a band of approximately 116 kDa (predicted molecular weight: 119 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
    • 組織特異性
      Expressed in both normal and transformed breast epithelial cell lines.
    • 配列類似性
      Contains 2 IBR-type zinc fingers.
      Contains 3 RanBP2-type zinc fingers.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • ドメイン
      The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E3 ubiquitin protein ligase RNF31 antibody
      • FLJ10111 antibody
      • FLJ23501 antibody
      • HOIL 1L interacting protein antibody
      • HOIL-1-interacting protein antibody
      • HOIL1L interacting protein antibody
      • HOIP antibody
      • RING finger protein 31 antibody
      • RNF31 antibody
      • RNF31_HUMAN antibody
      • ZIBRA antibody
      • Zinc in between ring finger ubiquitin associated domain antibody
      • Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
      see all

    画像

    • Anti-RNF31/HOIP antibody - ChIP Grade (ab46322) at 1/250 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 119 kDa
      Observed band size: 116 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 105 kDa (possible isoform), 50 kDa (possible isoform), 60 kDa. We are unsure as to the identity of these extra bands.

    • ICC/IF image of ab46322 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46322, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 cells at 5µg/ml.
    • Western blot analysis of A549 cell lysate (100μg/lane) RNF31/HOIP with ab46322 at 1/1000. A HRP-conjugated Goat anti-rabbit polyclonal (1/10000) was used as the secondary antibody.

      lane 1-2 : siCT A549
      lane 3-4 : siHOIP A549

      See Abreview

    参考文献

    This product has been referenced in:
    • Ginster S  et al. Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation. PLoS One 12:e0169026 (2017). Read more (PubMed: 28052131) »
    • Zhu J  et al. RING finger protein 31 promotes p53 degradation in breast cancer cells. Oncogene 35:1955-64 (2016). WB, IP . Read more (PubMed: 26148235) »

    See all 9 Publications for this product

    レビューと Q&A

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 Cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    100 µg
    Specification
    A549 Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 10 2018

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (primary mouse alveolar type II cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    125 µg
    Specification
    primary mouse alveolar type II cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 20 2017

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris, MES buffer)
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Specification
    Mouse embryonic fibroblasts
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Dr. Paola Marco I Casanova

    Verified customer

    投稿 Mar 28 2014

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris, MES buffer)
    Sample
    Human Cell lysate - whole cell (HEK293 and hTERT-RPE1)
    Specification
    HEK293 and hTERT-RPE1
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 28 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK 293)
    Loading amount
    30 µg
    Specification
    HEK 293
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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