製品の概要

  • 製品名Anti-RNF31/HOIP antibody
    RNF31/HOIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RNF31/HOIP
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1022-1072 of Human RNF31/HOIP (NP_060469.4).

  • ポジティブ・コントロール
    • HeLa whole cell lysate Jurkat whole cell lysate 293T whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 120 kDa.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
    • 組織特異性Expressed in both normal and transformed breast epithelial cell lines.
    • 配列類似性Contains 2 IBR-type zinc fingers.
      Contains 3 RanBP2-type zinc fingers.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • ドメインThe RanBP2-type zinc fingers mediate the specific interaction with ubiquitin.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E3 ubiquitin protein ligase RNF31 antibody
      • FLJ10111 antibody
      • FLJ23501 antibody
      • HOIL 1L interacting protein antibody
      • HOIL-1-interacting protein antibody
      • HOIL1L interacting protein antibody
      • HOIP antibody
      • RING finger protein 31 antibody
      • RNF31 antibody
      • RNF31_HUMAN antibody
      • ZIBRA antibody
      • Zinc in between ring finger ubiquitin associated domain antibody
      • Zinc in-between-RING-finger ubiquitin-associated domain protein antibody
      see all

    Anti-RNF31/HOIP antibody 画像

    • All lanes : Anti-RNF31/HOIP antibody (ab125189) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 120 kDa


      Exposure time : 3 minutes

    Anti-RNF31/HOIP antibody (ab125189) 使用論文

    ab125189 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Sample Human Cell lysate - whole cell (HEK293 and hTERT-RPE1)
    Specification HEK293 and hTERT-RPE1
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
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    投稿 Mar 31 2014

    Application Western blot
    Loading amount 10 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris, MES buffer)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Specification Mouse embryonic fibroblasts
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
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    Abcam user community

    Verified customer

    投稿 Mar 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"