製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF25
  • アプリケーション
    適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
IP Use a concentration of 1 µg/ml.
ELISA 1/10000 - 1/40000.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • ドメイン
    The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • 翻訳後修飾
    Ubiquitinated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AO7 antibody
    • E3 ubiquitin protein ligase RNF25 antibody
    • E3 ubiquitin-protein ligase RNF25 antibody
    • EC 6.3.2.- antibody
    • FLJ13906 antibody
    • RING finger protein 25 antibody
    • RING finger protein AO7 antibody
    • RNF 25 antibody
    • RNF25 antibody
    • RNF25_HUMAN antibody
    see all

画像

  • All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution

    Lane 1 : HEK293 lysate from cells transfected with human RNF25
    Lane 2 : HEK293 lysate from cells transfected with mouse RNF25
    Lane 3 : HEK293 lysate from cells transfected with empty vector


    Predicted band size : 51 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  • 1ug ab51475 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.

参考文献

ab51475 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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