製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF25
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids near the C terminus of Human RNF25.

  • ポジティブ・コントロール
    • Human Brain Cerebellum tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Predicted molecular weight: 51 kDa.
IP Use at an assay dependent concentration.
ELISA 1/10000 - 1/40000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 RING-type zinc finger.
    Contains 1 RWD domain.
  • ドメイン
    The RING-type zinc finger domain interacts with an ubiquitin-conjugating enzyme (E2) and facilitates ubiquitination.
  • 翻訳後修飾
    Ubiquitinated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AO7 antibody
    • E3 ubiquitin protein ligase RNF25 antibody
    • E3 ubiquitin-protein ligase RNF25 antibody
    • EC 6.3.2.- antibody
    • FLJ13906 antibody
    • RING finger protein 25 antibody
    • RING finger protein AO7 antibody
    • RNF 25 antibody
    • RNF25 antibody
    • RNF25_HUMAN antibody
    see all

画像

  • ab115543, at 5µg/ml, staining RNF25 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

参考文献

ab115543 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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