Anti-RNF220 抗体 (ab69357)
Key features and details
- Mouse polyclonal to RNF220
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RNF220 antibody
RNF220 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to RNF220 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human RNF220. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- RNF220 transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69357の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 63 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 63 kDa). |
ターゲット情報
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関連性
RNF220 contains one RING-type zinc finger. There are two named isoforms. -
参照データベース
- Entrez Gene: 55182 Human
- SwissProt: Q5VTB9 Human
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別名
- C1orf164 antibody
- Chromosome 1 open reading frame 164 antibody
- FLJ10597 antibody
see all
画像
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All lanes : Anti-RNF220 antibody (ab69357) at 1/500 dilution
Lane 1 : RNF220 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 63 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
ab69357 at 10µg/ml staining RNF220 in Hela cells by ICC/IF.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab69357 は 1 報の論文で使用されています。
- Yan J et al. Ring finger 220 promotes the stemness and progression of colon cancer cells via Ubiquitin specific peptidase 22-BMI1 axis. Bioengineered 12:12060-12069 (2021). PubMed: 34753387