Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling RNF22 with ab74189 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - RNF22 antibody (ab74189)
Detection of RNF22 by Western Blot of Immunprecipitate.
anit-RNF22 (ab74191), at 1µg/ml, staining RNF22 in HeLa whole cell lysate immunoprecipitated using ab74189 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.