製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF22
  • アプリケーション
    適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human RNF22 (NP_006449.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab74189 was affinity purified using an epitope specific to RNF22 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
    • 組織特異性
      Expressed in brain, heart, uterus and testis.
    • 配列類似性
      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 filamin repeat.
      Contains 6 NHL repeats.
      Contains 1 RING-type zinc finger.
    • ドメイン
      The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
    • 細胞内局在
      Cytoplasm. Early endosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BERP antibody
      • Brain expressed ring finger antibody
      • Brain-expressed RING finger protein antibody
      • FLJ16135 antibody
      • HAC1 antibody
      • RING finger protein 22 antibody
      • RING finger protein 97 antibody
      • RNF22 antibody
      • RNF97 antibody
      • Trim3 antibody
      • TRIM3_HUMAN antibody
      • Tripartite motif containing 3 antibody
      • Tripartite motif protein TRIM3 antibody
      • Tripartite motif-containing protein 3 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling RNF22 with ab74189 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of RNF22 by Western Blot of Immunprecipitate.
      anit-RNF22 (ab74191), at 1µg/ml, staining RNF22 in HeLa whole cell lysate immunoprecipitated using ab74189 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    参考文献

    ab74189 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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