製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RNF22
  • 特異性
    This antibody is expected to recognise both human isoforms.
  • アプリケーション
    適用あり: IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide:

    AKREDSPGPEVQP-C

    , corresponding to N terminal amino acids 2-14 of Human RNF22 (Peptide available as ab23010.)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
WB
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Approx 85kDa band seen in Mouse Brain and Human Brain (Cerebellum) lysate [Predicted MW of approx. 80.8kDa according to NP_006449.2].


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
    • 組織特異性
      Expressed in brain, heart, uterus and testis.
    • 配列類似性
      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 filamin repeat.
      Contains 6 NHL repeats.
      Contains 1 RING-type zinc finger.
    • ドメイン
      The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
    • 細胞内局在
      Cytoplasm. Early endosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BERP antibody
      • Brain expressed ring finger antibody
      • Brain-expressed RING finger protein antibody
      • FLJ16135 antibody
      • HAC1 antibody
      • RING finger protein 22 antibody
      • RING finger protein 97 antibody
      • RNF22 antibody
      • RNF97 antibody
      • Trim3 antibody
      • TRIM3_HUMAN antibody
      • Tripartite motif containing 3 antibody
      • Tripartite motif protein TRIM3 antibody
      • Tripartite motif-containing protein 3 antibody
      see all

    画像

    • All lanes : Anti-RNF22 antibody (ab4215) at 0.3 µg/ml

      Lane 1 : Human cerebellum lysate (RIPA Buffer)
      Lane 2 : Rat brain lysate (RIPA Buffer)

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 81 kDa
    • Anti-RNF22 antibody (ab4215) at 0.5 µg/ml + Mouse Brain (RIPA buffer) at 30 µg

      Predicted band size : 81 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
    • ab4215 immunoprecipitating RNF22 from rat brain tissue whole lysate. The antibody was used at a concentration of 2.5ug/mg of lysate. A Protein G matrix was used. The same antibody was used in the Western blot stage of the experiment (left hand panel).

      See Abreview

    参考文献

    This product has been referenced in:
    • Schreiber J  et al. Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic ?-actin levels. J Cell Biol 211:569-86 (2015). Read more (PubMed: 26527743) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (Brain)
    Total protein in input
    500 µg
    Specification
    Brain
    Immuno-precipitation step
    Protein G
    Username

    忠義 大塚

    Verified customer

    投稿 Jun 27 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Brain)
    Loading amount
    30 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    忠義 大塚

    Verified customer

    投稿 Jun 27 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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