製品の概要

  • 製品名Anti-RNF20 antibody
    RNF20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RNF20
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 125 and 175 of human RNF20. (NP_062538.5)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
    • 組織特異性Expressed in the normal brain and also in malignant gliomas (at protein level).
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the BRE1 family.
      Contains 1 RING-type zinc finger.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BRE1 A antibody
      • BRE1 antibody
      • BRE1 E3 ubiquitin ligase homolog antibody
      • BRE1-A antibody
      • BRE1A antibody
      • BRE1A_HUMAN antibody
      • E3 ubiquitin-protein ligase BRE1A antibody
      • hBRE1 antibody
      • Homolog of S. cerevisiae BRE1 antibody
      • RING finger protein 20 antibody
      • Ring finger protein 20 E3 ubiquitin protein ligase antibody
      • Rnf20 antibody
      see all

    Anti-RNF20 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling RNF20 with ab70496 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-RNF20 antibody (ab70496) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 114 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of Human RNF20 by Western Blot of Immunprecipitate. ab70496 at 1µg/ml staining RNF20 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using ab70496. Detection: Chemiluminescence with exposure times of 10 seconds.

    Anti-RNF20 antibody (ab70496) 使用論文

    ab70496 has not yet been referenced specifically in any publications.

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