製品の概要

  • 製品名Anti-RNF20 antibody
    RNF20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RNF20
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human RNF20

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).
IP Use at 2 µg/mg of lysate.
ICC/IF 1/1000.

ターゲット情報

  • 機能Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • 組織特異性Expressed in the normal brain and also in malignant gliomas (at protein level).
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

Anti-RNF20 antibody 画像

  • All lanes : Anti-RNF20 antibody (ab70495) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 114 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 171 kDa,280 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human RNF20 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70495 at 3 µg/mg lysate. Subsequent RNF20 WB detection was performed using ab70495 at 1 µg/ml.
  • ab70495 (1/1000) detecting RNF20 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.

    See Abreview

Anti-RNF20 antibody (ab70495) 使用論文

This product has been referenced in:
  • Buro LJ  et al. Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon regulatory factor 1 gene (IRF1). Epigenetics Chromatin 3:16 (2010). WB ; Human . Read more (PubMed: 20825659) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Feb 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"