製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF20
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from within residues 100 - 200 of Human RNF20.

    (Peptide available as ab27758.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human hTERT fibroblasts whole cell lysate within Western blot and HeLa whole cell lysate within Immunofluorescence.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.

Use with methanol fixed cells. This antibody does not appear to be compatible with paraformaldehyde fixation.

WB 1/500.

ab33500 can show some non-specific bands - anyone wanting to use this antibody for WB should consider image below or abreview.

ターゲット情報

  • 機能
    Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • 組織特異性
    Expressed in the normal brain and also in malignant gliomas (at protein level).
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the BRE1 family.
    Contains 1 RING-type zinc finger.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRE1 A antibody
    • BRE1 antibody
    • BRE1 E3 ubiquitin ligase homolog antibody
    • BRE1-A antibody
    • BRE1A antibody
    • BRE1A_HUMAN antibody
    • E3 ubiquitin-protein ligase BRE1A antibody
    • hBRE1 antibody
    • Homolog of S. cerevisiae BRE1 antibody
    • RING finger protein 20 antibody
    • Ring finger protein 20 E3 ubiquitin protein ligase antibody
    • Rnf20 antibody
    see all

画像

  • ab33500 (1/500) detecting RNF20 in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

  • All lanes : Anti-RNF20 antibody (ab33500) at 1/500 dilution

    Lane 1 : 20 µg Human hTERT fibroblasts whole cell lysate
    Lane 2 : 20 µg Human hTERT fibroblasts whole cell lysate transfected with RNF20 siRNA (3 days)

    Secondary
    All lanes : Goat anti-Rabbit conjugated to HRP at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 150 kDa (possible non-specific binding), 170 kDa (possible non-specific binding), 60 kDa (possible non-specific binding)

    See Abreview

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
25 µg
Specification
U2OS
Treatment
Control or RNF40 (not RNF20) siRNA for 72 h.
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Prof. Steven Johnsen

Verified customer

投稿 Aug 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (hTERT fibroblasts)
Loading amount
30 µg
Specification
hTERT fibroblasts
Treatment
RNF20 siRNA for 3 days
Gel Running Conditions
Reduced Denaturing (7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Jul 15 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Feb 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231 and HeLa Cells)
Specification
MDA-MB-231 and HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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