製品の概要

  • 製品名
    Anti-RNF187 antibody [5H10]
    RNF187 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5H10] to RNF187
  • アプリケーション
    適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Single C-terminal immunogenic peptide

  • エピトープ
    aa174-189.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    5H10
  • ミエローマ
    Sp2
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab105034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that acts as a coactivator of JUN-mediated gene activation in response to growth factor signaling via the MAP3K1 pathway, independently from MAPK8.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 RING-type zinc finger.
  • ドメイン
    The RING-type zinc finger domain is required for E3 ligase activity.
  • 翻訳後修飾
    Ubiquitinated; undergoes 'Lys-48'-linked autoubiquitination in the absence of growth factors and MAP3K1-induced 'Lys-63'-linked polyubiquitination. 'Lys-48'-autoubiquitination leads to degradation by the proteasome, while MAP3K1-induced 'Lys-63'-linked polyubiquitination results in the stabilization of the protein. 'Lys-48'- and 'Lys-63'-linked polyubiquitinations occur most probably on the same 3 C-terminal lysine residues (Lys-195, Lys-223 and Lys-224) and are thus mutually exclusive. Other sites of ubiquitination are not excluded.
    Arginine methylation by PRMT1 stabilizes RNF187 by facilitating K63-linked ubiquitin chain formation, and enables dimerization, c-Jun interaction and subsequent AP1 target gene expression.
  • 細胞内局在
    Cytoplasm. Nucleus. Predominantly located in the cytoplasm. Shuttles between the cytoplasm and the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase RNF187 antibody
    • Protein RNF187 antibody
    • RACO-1 antibody
    • RACO1 antibody
    • RING domain AP1 coactivator 1 antibody
    • RING finger protein 187 antibody
    • RN187_HUMAN antibody
    • RNF187 antibody
    see all

参考文献

This product has been referenced in:
  • Davies CC  et al. Identification of a co-activator that links growth factor signalling to c-Jun/AP-1 activation. Nat Cell Biol 12:963-72 (2010). Read more (PubMed: 20852630) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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