Anti-RNF144A 抗体 (ab89260)
Key features and details
- Mouse polyclonal to RNF144A
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RNF144A antibody
RNF144A 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to RNF144A -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein within Human RNF144A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_055561.2 -
ポジティブ・コントロール
- Rat brain tissue lysate, HeLa whole cell lysate (ab150035), RNF144A transfected 293T cell lysate and HeLa cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89260の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the RBR family. RNF144 subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9781 Human
- Entrez Gene: 500636 Rat
- SwissProt: P50876 Human
- Unigene: 22146 Human
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別名
- KIAA0161 antibody
- Probable E3 ubiquitin protein ligase RNF144A antibody
- Probable E3 ubiquitin-protein ligase RNF144A antibody
see all
画像
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Anti-RNF144A antibody (ab89260) at 5 µg/ml + Rat brain tissue lysate at 50 µg
Predicted band size: 33 kDa -
Anti-RNF144A antibody (ab89260) at 5 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 33 kDa -
All lanes : Anti-RNF144A antibody (ab89260) at 5 µg/ml
Lane 1 : RNF144A transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 33 kDa -
ab89260 at 10µg/ml staining RNF144A on HeLa cell by immunofluorescence
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab89260 は 5 報の論文で使用されています。
- Han SH & Kim KT RNF144a induces ERK-dependent cell death under oxidative stress via downregulation of vaccinia-related kinase 3. J Cell Sci 133:N/A (2020). PubMed: 33067254
- Ho SR & Lin WC RNF144A sustains EGFR signaling to promote EGF-dependent cell proliferation. J Biol Chem 293:16307-16323 (2018). PubMed: 30171075
- Jin X et al. Targeting glioma stem cells through combined BMI1 and EZH2 inhibition. Nat Med 23:1352-1361 (2017). WB ; Human . PubMed: 29035367
- Ho SR et al. Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain. J Biol Chem 290:23026-38 (2015). PubMed: 26216882
- Ho SR et al. RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A 111:E2646-55 (2014). PubMed: 24979766