製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF138
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to an internal sequence of Human RNF138.

  • ポジティブ・コントロール
    • MCF7 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Contains 1 RING-type zinc finger.
    • ドメイン
      The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    • 翻訳後修飾
      Auto-ubiquitinated.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E3 ubiquitin protein ligase RNF138 antibody
      • E3 ubiquitin-protein ligase RNF138 antibody
      • hNARF antibody
      • HSD 4 antibody
      • HSD4 antibody
      • MGC8758 antibody
      • NARF antibody
      • Nemo like kinase associated RING finger protein antibody
      • Nemo-like kinase-associated RING finger protein antibody
      • NLK associated RING finger protein antibody
      • NLK-associated RING finger protein antibody
      • RING finger protein 138 antibody
      • ring finger protein 138, E3 ubiquitin protein ligase antibody
      • RN138_HUMAN antibody
      • Rnf138 antibody
      • STRIN antibody
      see all

    画像

    • All lanes : Anti-RNF138 antibody (ab92730) at 1/500 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : MCF7 cell lysate with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa

    プロトコール

    参考文献

    ab92730 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Sample
    Mouse Tissue lysate - whole (Multiple Tissues)
    Specification
    Multiple Tissues
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Oct 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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