製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RNF13
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: PNGERDYNIANTV, corresponding to C terminal amino acids 369-381 of Human RNF13.

  • ポジティブ・コントロール
    • Human pancreas.
  • 特記事項
    Principal Names – RNF13; RZF; ring finger protein 13; RING zinc finger protein. Official Gene Symbol - RNF13. GenBank Accession Number – NP_009213. LocusLink ID - 11342 (human). Gene Ontology terms - peptidase activity; proteolysis and peptidolysis; nucleus.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. This antibodoidy stained islets but not in ducts and acini of Human normal pancreas. However, in pancreatic ductal adenocarcinoma staining was in the ducts and acini.
WB Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human RNF13 peptide (ab23020).

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that may play a role in controlling cell proliferation.
  • 組織特異性
    Widely expressed (at protein level). In normal pancreas, expressed in islets, but not in ducts, nor in acini (at protein level).
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 PA (protease associated) domain.
    Contains 1 RING-type zinc finger.
  • ドメイン
    The RING-type zinc finger domain is required for E3 ligase activity.
  • 翻訳後修飾
    Auto-ubiquitinated.
  • 細胞内局在
    Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome membrane. Lysosome membrane. Nucleus inner membrane. Under certain conditions, relocalizes to recycling endosomes and to the inner nuclear membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase RNF13 antibody
    • FLJ93817 antibody
    • MGC13689 antibody
    • RING finger protein 13 antibody
    • Ring zinc finger protein antibody
    • RNF 13 antibody
    • Rnf13 antibody
    • RNF13_HUMAN antibody
    • RZF antibody
    see all

Anti-RNF13 antibody (ab4288) 使用論文

This product has been referenced in:
  • Zhang Q  et al. RNF13: a novel RING-type ubiquitin ligase over-expressed in pancreatic cancer. Cell Res 19:348-57 (2009). WB, IHC-P ; Human . Read more (PubMed: 18794910) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - Triton
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Miss. Chen Avrahami

Verified customer

投稿 May 16 2016

Thank you very much for contacting us regarding ab4288. I am very sorry to hear that you are experiencing difficulty with this antibody. Where did you place the tag on RNF13? It is possible that the tag is interfering with your ability to detect t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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