製品の概要

  • 製品名Anti-RNF121 antibody
  • 製品の詳細
    Rabbit polyclonal to RNF121
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: LFSAVTAFVT FRATRKPLVQ TTPRLVYKWF LLIYKISYAT GIVGYMAVMF, corresponding to amino acids 110-159 of Human RNF121

  • ポジティブ・コントロール
    • Jurkat cell lysate and human intestine tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ターゲット情報

  • 関連性RNF121 protein contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. Three of them are supported by at least two independent transcripts or ESTs, the full length natures of others are not clear.
  • 細胞内局在Membrane; Multi-pass membrane protein
  • 参照データベース
  • 別名
    • 4930544L10Rik antibody
    • FLJ11099 antibody
    • ring finger protein 121 antibody

Anti-RNF121 antibody 画像

  • Anti-RNF121 antibody (ab59152) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling RNF121 with ab59152 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnifiaction: 400X.

Anti-RNF121 antibody (ab59152) 使用論文

ab59152 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"