製品の概要

  • 製品名Anti-RNF12 antibody
    RNF12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RNF12
  • 特異性ab22813 recognises RNF12.
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide derived from a region between residues 530-584 of Human RNF12.

  • ポジティブ・コントロール
    • Jurkat cell lysate and Human spermatophore tissue sections.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab22813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.
  • 組織特異性Expressed in many tissues.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the RNF12 family.
    Contains 1 RING-type zinc finger.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase RLIM antibody
    • LIM domain-interacting RING finger protein antibody
    • R-LIM antibody
    • Renal carcinoma antigen NY-REN-43 antibody
    • RING finger LIM domain-binding protein antibody
    • RING finger protein 12 antibody
    • RLIM antibody
    • RNF12 antibody
    • RNF12_HUMAN antibody
    see all

Anti-RNF12 antibody 画像

  • ab22813 used at 4-8 µg/ml on parffin embeded tissue: Human spermatophore cells with positive label: Epithelial cells (indicated with arrows)
  • Anti-RNF12 antibody (ab22813) at 1 µg/ml + Jurkat cell lysate
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

Anti-RNF12 antibody (ab22813) 使用論文

ab22813 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"