製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RNF113A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 287-336 (QHFRTTPRCY VCDQQTNGVF NPAKELIAKL EKHRATGEGG ASDLPEDPDE) of Human RNF113A (NP_008909).

  • ポジティブ・コントロール
    • Transfected 293T nuclear lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    RNF113A (Ring finger protein 113A) is a novel gene whose function cannot directly be inferred from it's sequence analysis. Ring finger proteins have a role in signalling the destruction of a range of heterologous protein substrates. They are small zinc binding domains present within arrays of larger, functionally distinct proteins, often close to the amino or carboxyl termini. RNF113A is a ubiquitously expressed protein that contains a RING type zinc finger and a C3H1 type zinc finger.
  • 参照データベース
  • 別名
    • Cwc24 antibody
    • Ring finger protein 113A antibody
    • RNF 113A antibody
    • RNF113 antibody
    • Zinc finger protein 183 antibody
    • ZNF183 antibody
    see all

画像

  • Anti-RNF113A antibody (ab85797) at 1 µg/ml (5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab85797 has not yet been referenced specifically in any publications.

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