The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 109 kDa.
Use a concentration of 3 µg/ml.
Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.
Protein modification; protein ubiquitination.
Contains 1 RING-type zinc finger.
Nucleus. Cytoplasm. Upon TGF-beta treatment, translocates from nucleus to cytosol.