The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 109 kDa.
Use a concentration of 3 µg/ml.
機能Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.
パスウェイProtein modification; protein ubiquitination.
配列類似性Contains 1 RING-type zinc finger.
細胞内局在Nucleus. Cytoplasm. Upon TGF-beta treatment, translocates from nucleus to cytosol.