製品の概要

  • 製品名Anti-RNF111 antibody
    RNF111 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RNF111
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-109 of Human RNF111

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.
    • 組織特異性Broadly expressed.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Contains 1 RING-type zinc finger.
    • 細胞内局在Nucleus. Cytoplasm. Upon TGF-beta treatment, translocates from nucleus to cytosol.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ARK antibody
      • Arkadia antibody
      • DKFZp313E0731 antibody
      • DKFZp686H1966 antibody
      • DKFZp761D081 antibody
      • E3 ubiquitin protein ligase Arkadia antibody
      • E3 ubiquitin-protein ligase Arkadia antibody
      • FLJ38008 antibody
      • RING finger protein 111 antibody
      • RN111_HUMAN antibody
      • RNF 111 antibody
      • RNF111 antibody
      see all

    Anti-RNF111 antibody 画像

    • Western blot against tagged recombinant protein immunogen using ab57416 RNF111 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

    Anti-RNF111 antibody (ab57416) 使用論文

    ab57416 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (aortic smooth muscle cell)
    Loading amount 20 µg
    Specification aortic smooth muscle cell
    Gel Running Conditions Reduced Denaturing (3-8% Tris-Acetate)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Mar 12 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"