製品の概要

  • 製品名Anti-RNF11 antibody
    RNF11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RNF11
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Rabbit, Guinea pig, Cow, Cat, Human
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 40-89 (SDDISLLHES QSDRASFGEG TEPDQEPPPP YQEQVPVPVY HPTPSQARLA) of Rat RNF11 (XM_001067720).

  • ポジティブ・コントロール
    • Rat liver lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.
  • 組織特異性Expressed at low levels in the lung, liver, kidney, pancreas, spleen, prostate, thymus, ovary, small intestine, colon, and peripheral blood lymphocytes, and, at intermediate levels, in the testis, heart, brain and placenta. Highest expression in the skeletal muscle. In the brain, expressed at different levels in several regions: high levels in the amygdala, moderate in the hippocampus and thalamus, low in the caudate and extremely low levels in the corpus callosum (at protein level). Restricted to neurons, enriched in somatodendritic compartments and excluded from white matter (at protein level). In substantia nigra, present in cell bodies and processes of dopaminergic and nondopaminergic cells (at protein level). In Parkinson disease, sequestered in Lewy bodies and neurites. Overexpressed in breast cancer cells, but not detected in the surrounding stroma and weakly, if at all, in normal breast epithelial cells (at protein level). Also expressed in several tumor cell lines.
  • 配列類似性Contains 1 RING-type zinc finger.
  • ドメインThe WW-binding motif mediates interaction with NEDD4.
  • 翻訳後修飾Ubiquitinated in the presence of SMURF2 and UBE2D1, as well as WWP1.
    Phosphorylation by PKB/AKT1 may accelerate degradation by the proteasome.
  • 細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic, when unphosphorylated, and nuclear, when phosphorylated by PKB/AKT1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CGI 123 antibody
    • RING finger protein 11 antibody
    • RNF11 antibody
    • RNF11_HUMAN antibody
    • Sid 1669 antibody
    • SID1669 antibody
    see all

Anti-RNF11 antibody 画像

  • Anti-RNF11 antibody (ab123140) at 1 µg/ml + Rat liver lysate at 10 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa

Anti-RNF11 antibody (ab123140) 使用論文

ab123140 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Human Cell lysate - whole cell (HCT116 colon carcinoma)
Specification HCT116 colon carcinoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Feb 18 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela, HEK293)
Loading amount 30 µg
Specification Hela, HEK293
Gel Running Conditions Reduced Denaturing (13,5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jun 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"