Anti-RNA polymerase II RPB1 抗体 [EPR1509Y] - ChIP Grade (ab76123)

製品の概要

  • 製品名Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade
    RNA polymerase II RPB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1509Y] to RNA polymerase II RPB1 - ChIP Grade
  • アプリケーション適用あり: WB, ICC, Flow Cyt, ChIPmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N terminus of human RNA polymerase II

  • ポジティブ・コントロール
    • HeLa cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 217 kDa).
ICC 1/50.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP Use at an assay dependent concentration. PubMed: 22249179
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 関連性RPB1 is a subunit of RNA polymerase II which catalyses transcription of RNA from DNA. RPB1 forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.
    • 細胞内局在Nuclear
    • 参照データベース
    • 別名
      • DNA directed RNA polymerase II subunit RPB1 antibody
      • hRPB220 antibody
      • hsRPB1 antibody
      • POLR2 antibody
      • POLR2A antibody
      • POLRA antibody
      • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody
      • RNA polymerase II subunit 1 antibody
      • RNA polymerase II subunit B1 antibody
      • RP02 antibody
      • RPB220 antibody
      • RPBh1 antibody
      • RpIILS antibody
      • RPO21 antibody
      • RPOL2 antibody
      • SUA8 antibody
      see all

    Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade 画像

    • Overlay histogram showing HeLa cells stained with ab76123 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76123, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade (ab76123) at 1/2000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 217 kDa
      Observed band size : 270 kDa (why is the actual band size different from the predicted?)

    Anti-RNA polymerase II RPB1 antibody [EPR1509Y] - ChIP Grade (ab76123) 使用論文

    This product has been referenced in:
    • Kim JY  et al. Chronic ethanol consumption inhibits glucokinase transcriptional activity by Atf3 and triggers metabolic syndrome in vivo. J Biol Chem 289:27065-79 (2014). ChIP . Read more (PubMed: 25074928) »
    • Kim JW  et al. New molecular bridge between RelA/p65 and NF-?B target genes via histone acetyltransferase TIP60 cofactor. J Biol Chem 287:7780-91 (2012). ChIP ; Human . Read more (PubMed: 22249179) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"