製品の概要

  • 製品名Anti-RNA Helicase A antibody
    RNA Helicase A 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to RNA Helicase A
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein:

    DLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVV PWSPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQA

    , corresponding to amino acids 281/380 of Human RNA Helicase A

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 141 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability.
  • 配列類似性Belongs to the DEAD box helicase family. DEAH subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • ドメインThe MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1.
  • 翻訳後修飾Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
    May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP dependent RNA helicase A antibody
    • ATP-dependent RNA helicase A antibody
    • DDX 9 antibody
    • DDX9 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 9 antibody
    • DEAD/H box 9 antibody
    • DEAD/H box polypeptide 9 antibody
    • DEAH (Asp Glu Ala His) box polypeptide 9 antibody
    • DEAH box polypeptide 9 antibody
    • DEAH box protein 9 antibody
    • DHX 9 antibody
    • dhx9 antibody
    • DHX9_HUMAN antibody
    • Leukophysin antibody
    • LKP antibody
    • NDH 2 antibody
    • NDH II antibody
    • NDH2 antibody
    • NDHII antibody
    • Nuclear DNA helicase II antibody
    • RHA antibody
    see all

Anti-RNA Helicase A antibody 画像

  • All lanes : Anti-RNA Helicase A antibody (ab22174) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 141 kDa

Anti-RNA Helicase A antibody (ab22174) 使用論文

ab22174 has not yet been referenced specifically in any publications.

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