製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RMI2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human RMI2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both HEK293 and MCF7 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).

ターゲット情報

  • 機能
    Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. The complex is therefore essential for the stability, localization, and function of complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
  • 配列類似性
    Belongs to the RMI2 family.
    Contains 1 OB DNA-binding domain.
  • 翻訳後修飾
    Phosphorylated during mitosis.
  • 細胞内局在
    Nucleus. Colocalizes with BLM at nuclear DNA repair foci.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BLAP18 antibody
    • BLM-associated protein of 18 kDa antibody
    • C16orf75 antibody
    • hRMI2 antibody
    • MGC24665 antibody
    • RecQ-mediated genome instability protein 2 antibody
    • RMI2 antibody
    • RMI2_HUMAN antibody
    see all

画像

  • All lanes : Anti-RMI2 antibody (ab108491) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 17 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    The predicted molecular weight of RMI2 is 15 kDa (SwissProt), however we expect to observe a banding pattern around 18 kDa. Abcam recommends using milk as the blocking agent (3%). This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab108491 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

プロトコール

参考文献

ab108491 has not yet been referenced specifically in any publications.

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