製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RLF
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 500-550 of Human RLF (Q13129).

  • ポジティブ・コントロール
    • HeLa and 293T cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 218 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

画像

  • All lanes : Anti-RLF antibody (ab115154) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg


    Predicted band size : 218 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of RLF (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-RLF antibody (ab115154); Lane 2- Immunoprecipitation with IgG control. Anti-RLF antibody (ab115154) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.

参考文献

ab115154 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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