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Tissue/ cell preparation: Rabbit leukemic B cell line.
In normal adult rabbits, this antibody has been detected in 50-60% of peripheral blood leukocytes and splenocytes. The antibody has also been reported to stain: lymphocytes in the thymic medulla and some cells in the cortex; periportal cells in the liver; and synovial lining cell (i.e., infiltrating macrophages) in inflamed synovium. DR transcripts have been detected in appendix, bone marrow, spleen and, to a lesser extent, in lymph nodes, lung, liver, and other organs.
Our Abpromise guarantee covers the use of ab25527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration.
|IP||Use at an assay dependent concentration.|
ab25527 has not yet been referenced specifically in any publications.